![]() Additional data should be considered for a more accurate adjustment.Ġ.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.ĥμl of IRIS9 Plus Prestained Protein Ladder (PMI09) resolves 9 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane. The WesternSure chemiluminescent protein ladder is the only pre-stained, multi-colored protein ladder for both film and digital chemiluminescence detection. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Precision Plus Protein Kaleidoscope Standards are a mixture of ten multicolored recombinant proteins (10250 kD). 2.5 μl per well for general Western transferring. Place this handy selection guide magnet on your refrigerator or lab bench to eliminate the guesswork and make protein standard selection simple. Refer to the IRIS9 Plus Prestained Protein Ladder patterns in various electrophoresis conditions:ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. The ladder is supplied in the gel loading buffer and is ready to use. Guide for Molecular Weight Estimation (kDa) Using a prestained protein ladder also helps researchers achieve accurate results more quickly due to its predictable and evenly distributed bands when run on an agarose gel or western blot. The Blue Ultra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. ![]() The IRIS9 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. There are two categories of commercially available protein ladders, unstained protein ladders and prestained protein ladders. The 9 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 15 kDa, a red band at 70 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. ![]() The IRIS9 Plus Prestained Protein Ladder is a combination of 9 pre-stained proteins with molecular weights from 15 to 170 kDa. ![]()
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